Research Assistant 3 #9727

Case Western Reserve University   Cleveland , OH   Full-time     Research & Development
Posted on April 19, 2022
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Working under limited supervision, the Research Assistant 3 will coordinate research work in the lab as part of a collaborative, multi-faceted project in the Division of Hematology/Oncology, focusing on the role of 15-Hydroxprostaglandin dehydrogenase (15-PGDH) in cancer and as a therapeutic target for tissue regeneration after injury. This extensive project requires the utilization of advanced skills in the techniques of molecular biology, cell biology and mouse studies. The research assistant will report weekly progress to the principal investigator and coordinate efforts with other workers. This position will work with mice.





  1. Coordinate and perform laboratory/research activities to sustain the appropriate levels of various mouse strains, maintenance of these strains and establishment of cell lines from various GI tumors and cell This includes, but is not limited to, providing care for animals, inspecting animals regularly, maintaining ID/records of all animals and keeping datasheets up-to-date and circulated appropriately. (50%)
  2. Work closely with the principal investigator for conducting a series of experiments to supply data as part of a larger scientific project on cancer genetics Design, manage and carry out complex and innovative research techniques, evaluate the efficaciousness of new techniques, recommend and develop new assays, analyze and formally present the resulting data before members of the laboratory on a regular basis. (15%)
  3. Devise new protocols and techniques for research projects involving high degree of skill and Adapt new techniques from literature to the lab. Collect, analyze and interpret data from multiple experiments. (15%)
  4. Evaluate adequacy of techniques; study and test new procedures and analyze Perform mammalian cell culture to establish and maintain the colon cancer cell line and xenograft banks; quantitate cell growth following established protocols and conduct transfection protocols involving human colon cells. (10%)





  1. May co-author research Perform literature searches independently when need dictates. May monitor lab budget. (5%)
  2. May supervise and train students and (5%)


  1. Perform laboratory maintenance tasks as Perform other duties as assigned. (<1%)





Department: Weekly contact with the laboratory principal investigator, is anticipated to discuss research and maintain workflow. Daily contact with research fellows overseeing 15-PGDH research projects.


University: Regular contact within the Department of Genetics or the Case Comprehensive Cancer Center are anticipated weekly to monthly to discuss research and maintain workflow.


External: Occasional contact with vendors as needed.


Students: Regular contact with students, research fellows and other technicians are expected to discuss research and maintain workflow.





May be asked to instruct staff or students new to the laboratory on techniques or common laboratory protocols.





Experience: 3 to 5 years of experience in biology or biochemistry required. Education: Bachelor of Science degree required.




  1. Has knowledge of commonly used concepts, practices and procedures within the cancer genetics field.
  2. Strong molecular biology skills (PCR, Western blots, basic cloning/subcloning techniques).
  3. Extensive experience with PCR, basic cloning/subcloning techniques, Western blotting and mouse studies.
  4. Strong working knowledge of human genetics and basic statistical
  5. Ability to interact with other lab personnel to coordinate laboratory activities, discuss experiments and carry out projects in accordance with general plans is essential. Ability to


work effectively, independently and collaboratively within a team (must be highly motivated, responsible, dependable and a self-starter).

  1. Computer skills related to data analysis and preparation of figures for
  2. Must demonstrate compliance with Case Western Reserve University Animal Research and Care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.
  3. Must demonstrate compassion for animals within Case Western Reserve University facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.
  4. Previous experience working with animals, particularly mice, is strongly
  5. Ability to meet consistent
  6. Ability to interact with colleagues, supervisors and customers face to





The employee will work in a fully equipped molecular biology laboratory in the Case Comprehensive Cancer Center research laboratories. General laboratory environment. May have exposure to hazards. Personal protective equipment, including lab coat, gloves and goggles, will be provided to limit exposure to hazards. Potential hazards include exposure to radioactivity, carcinogens, formaldehyde, laboratory chemicals, cell lines established from human tumors and human tissue samples. The employee also has the possibility of exposure to hepatitis virus and would need to receive hepatitis virus vaccine prophylaxis. Since this work involves clinical human samples, confidentiality needs to be maintained.


Case Western Reserve University’s animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals." This position and all animal research personnel, are subject to internal compliance to Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.


Diversity Statement



In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.


Reasonable Accommodations



Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.



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